About ACES BUFFER (For Molecular Biology)
Details :
- N-(2-acetamido)-2-aminoethane sulphonic acid 5 gm
- C4H10N2O4S M.W. 182.20 25gm
- Assay(alkalimetric) min. 99.0%
- Identity(IR spectrum) passes test
- UV-Absorption max. 0.05%
- (250 nm; 2%; 1cm; water)
- Chloride(Cl) max. 0.05%
- Sulphate(SO4) max. 0.005%
- Heavy metals(as Pb) max. 0.0005%
- Na(Sodium) max. 0.005%
- Loss on drying(105oC) max. 0.2%
- Biological buffer, pka at 20oC 6.8-7.0
Optimal Performance in Molecular BiologyACES BUFFER is specifically designed to deliver consistent and reliable buffering capacity during molecular biology protocols. Its pKa of 6.9 ensures effective pH stabilization, crucial for sensitive techniques like electrophoresis. The buffer's high solubility in water and stringent purity (99%) support reproducible results in demanding laboratory applications.
Strict Quality and Purity StandardsManufactured under rigorous quality control, ACES BUFFER meets molecular biology grade standards. With heavy metals restricted to under 5 ppm and exceptionally low endotoxin levels (<0.25 EU/mg), this buffer supports applications where contamination control is imperative. Its high assay (99%) guarantees buffer accuracy for experiments requiring precise chemical environments.
Safe Handling and Convenient StorageACES BUFFER is supplied as a stable, white crystalline solid in moisture-resistant HDPE bottles. Users should employ standard laboratory protective equipment, avoiding contact with skin and eyes. The buffer remains stable for up to five years when stored properly at room temperature with the container tightly closed in a dry, ventilated area.
FAQ's of ACES BUFFER (For Molecular Biology):
Q: How should ACES BUFFER be dissolved for use in laboratory protocols?
A: ACES BUFFER is freely soluble in water. To prepare a buffer solution, weigh the required amount of powder and dissolve it completely in distilled or deionized water, adjusting the volume as needed for your specific application.
Q: What are the main applications of ACES BUFFER in molecular biology research?
A: ACES BUFFER is commonly utilized for buffer solution preparation, electrophoresis procedures, and general molecular biology research where stable pH control is critical.
Q: When should I use ACES BUFFER instead of other buffering agents?
A: ACES BUFFER is ideal when you require a near-neutral pH buffer with a pKa of 6.9, particularly for systems sensitive to pH variations, such as protein or nucleic acid studies during electrophoresis or other molecular assays.
Q: Where can ACES BUFFER be safely stored, and what are the storage requirements?
A: Store ACES BUFFER at room temperature in its original HDPE bottle, keeping the container tightly sealed in a dry and well-ventilated place to maintain product stability.
Q: What safety precautions should be taken when handling ACES BUFFER?
A: Always use personal protective equipment such as gloves, goggles, and a lab coat while handling ACES BUFFER. Avoid direct contact with skin or eyes and handle the chemical in accordance with laboratory safety protocols.
Q: What benefits does ACES BUFFER offer for sensitive experiments?
A: ACES BUFFER provides high purity (99%), low endotoxin levels, and minimal heavy metal contamination, ensuring minimal interference and reliable results in molecular biology applications.