About AMIDO BLACK 10B (For Molecular Biology)
AMIDO BLACK 10B
(Naphthalene Black 12B)
C.I.No. 20470 100 gm
C22H14N6Na2O9S2 M.W. 616.50
Dye content (titanometric) 80%
Absorption max. (in water) 617-620 nm
Loss on drying (110oC) max.
Suitability as redox indicator passes test
Suitability for electrophoresis passes test
(Colorimetric determination
of protein in milk Redox Indicator)
CAS No. 1064-48-8 Un No. NR TARIFF : 3204 90 00
R : 36/37/38 S : 26-36
Reliable Staining for Protein AnalysisAMIDO BLACK 10B is commonly utilized in molecular biology laboratories for its efficiency in protein staining during electrophoresis and histological workflows. Its high solubility in water and strong absorption at 618 nm ensure vivid staining, contributing to accurate experimental results. This reagent supports researchers in visualizing and quantifying proteins with clarity and reproducibility.
Safe and Simple Laboratory ReagentFeaturing an odorless and stable composition, AMIDO BLACK 10B is classified as non-hazardous and is not regulated as hazardous material for transport. Its ease of storage-room temperature, shielded from light and moisture-adds further convenience, making it accessible for dealers, exporters, suppliers, and other industry professionals across India and beyond.
FAQ's of AMIDO BLACK 10B (For Molecular Biology):
Q: How is AMIDO BLACK 10B used in molecular biology laboratories?
A: AMIDO BLACK 10B is primarily used as a staining dye for visualizing proteins during electrophoresis and histological procedures. Researchers apply it to gels or tissue samples, where it binds to proteins, allowing for easy identification and analysis.
Q: What is the benefit of using AMIDO BLACK 10B in protein staining applications?
A: The main benefit is its high affinity for proteins, which generates clear, contrasting bands or images after staining. This helps scientists accurately detect and quantify proteins in a sample, enhancing data reliability in experiments.
Q: When should AMIDO BLACK 10B be applied during the laboratory workflow?
A: AMIDO BLACK 10B is typically applied after electrophoresis or sample preparation on gels or tissues. Following separation, the dye is used to stain proteins, making them visible for subsequent analysis.
Q: Where should AMIDO BLACK 10B be stored to maintain its efficacy?
A: It should be stored at room temperature in a bottle, protected from light and moisture. Proper storage ensures the dye retains its staining properties for up to five years.
Q: What are the solubility and physical properties of AMIDO BLACK 10B?
A: AMIDO BLACK 10B appears as a dark blue to black powder and is highly soluble in water. It is odorless and stable under normal laboratory conditions, decomposing at approximately 210C.
Q: Is AMIDO BLACK 10B safe for regular laboratory handling and transport?
A: Yes, AMIDO BLACK 10B is considered non-hazardous under standard handling conditions and is not classified as hazardous material for shipping purposes.
Q: Can AMIDO BLACK 10B be used by different types of laboratory suppliers in India?
A: Absolutely. AMIDO BLACK 10B is available to dealers, exporters, manufacturers, retailers, suppliers, and traders throughout India for use in molecular biology and related applications.