About AMIDO BLACK 10B
CERTIFICATE OF ANALYSIS
| Name Of Item : AMIDO BLACK 10B | Formula : C22H14N6Na2O9S2 |
| (naphthalene black 12B) | |
| M.W. : 616.50 | Batch No. : A0259 |
| CAS NO. : 1064-48-8 | Cat. No. : AL0237 00100 |
| Type Of Test | Standard | Observed |
| | | |
| Description | Blackish crystalline powder | Blackish crystalline powder |
| | | |
| | | |
| Dye content | (Min.) 80% | 80.0% |
| Absorption maxi. (in water) | 617-620nm | 618nm |
| Loss on drying at 105oC | <10% | 9.5% |
| Suitability as redox indicator | Passes test | Passes test |
| Suitability for electrophoresi | Passes test | Passes test |
Results : The above product complies with LR grade
Specialized Biological Staining CapabilitiesAMIDO BLACK 10B is widely used across biological research and clinical laboratories for its protein-staining properties. It offers reliable visualization of proteins in polyacrylamide gels, tissue samples, and other biological matrices. As an analytical reagent, it provides clear differentiation essential for research and diagnostics.
Stable and Reliable for Laboratory EnvironmentsEngineered for laboratory use, AMIDO BLACK 10B maintains stability under normal conditions and offers a shelf life of up to two years if stored properly. Its solubility in water and consistent performance make it suitable for repeated use in research and industrial applications.
Versatile in Industrial and Research ApplicationsThis azo dye finds applications beyond staining-serving as a dye in various industrial processes and analytical testing. Its compatibility with many substrates and pH-sensitive properties make it a versatile choice for numerous scientific and industrial tasks.
FAQ's of AMIDO BLACK 10B:
Q: How should AMIDO BLACK 10B be stored for maximum shelf life?
A: AMIDO BLACK 10B should be kept in a tightly sealed bottle, placed in a cool and dry environment. Proper storage helps maintain the product's stability and ensures its usability for up to two years.
Q: What is the primary use of AMIDO BLACK 10B in laboratory settings?
A: Its principal application is as a special stain for biological tissues, particularly in the visualization of proteins during electrophoresis and histological analysis. It is also employed as an analytical reagent in research laboratories.
Q: When is AMIDO BLACK 10B most effective as a pH indicator?
A: It shows its best performance within the pH range of 5 to 7, making it suitable for staining and detection tasks that fall within neutral to mildly acidic aqueous conditions.
Q: Where is AMIDO BLACK 10B commonly applied beyond academic research?
A: Beyond academia, AMIDO BLACK 10B is used in various industries as an industrial dye, especially for coloring textiles, and in analytical sectors, such as environmental testing labs and quality control departments.
Q: What is the benefit of using AMIDO BLACK 10B over other dyes for staining?
A: AMIDO BLACK 10B offers high solubility in water, reliable staining intensity, and stable performance, making it dependable for clear visualization and reproducible results in biological and analytical protocols.
Q: How is AMIDO BLACK 10B typically used in research processes?
A: Researchers dissolve the powder in water to prepare a dye solution, which is then applied to tissue sections or polyacrylamide gels for staining proteins. After application, excess dye is washed away, revealing distinct patterns for analysis.