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Eosine Blue

Name: Eosine Blue
Brand: ALPHA CHEMIKA
Price: 11626 INR
Availability: InStock

Price 11626 INR/ Gram

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MOQ : 500 Grams

Eosine Blue Specification

Purity
99%

Ph Level
Acidic

Size
25g, 100g, 500g, 1kg bottles

Storage Instructions
Store in a tightly closed container, in a cool, dry, well-ventilated place, away from sources of ignition and strong oxidizers.

Physical State
Powder

Usage
Biological Stain, Laboratory Research

Molecular Weight
692.83 g/mol

Molecular Formula
C20H6Br4Na2O5

Shelf Life
36 months

Packaging Type
Bottle / HDPE / Glass bottle

Melting Point
~220°C (decomposes)

CAS No
548-24-3)

Grade
Laboratory Grade

Type
University Lab Chemicals

Application
Biological Staining, Microscopy, Histological Staining

Appearance
Brownish Red Crystalline Powder

Purity(%)
99%

Synonyms
Eosin Y, Eosin Bluish

Staining Principle
Acid stain used for cytoplasmic and connective tissue staining

Hazard Class
Not classified as hazardous under normal laboratory conditions

Solubility
Soluble in water, slightly soluble in alcohol

EC Number
208-253-0

Odor
Odorless

Quality Standard
Meets analytical reagent standards

Color Index
45380

Eosine Blue Trade Information

Minimum Order Quantity
500 Grams

Supply Ability
50000 Grams Per Week

Delivery Time
4-5 Days

Sample Available
Yes

Sample Policy
Sample costs shipping and taxes has to be paid by the buyer

Packaging Details
500GM, 1KG, 2.5KG, 5KG, 25KG, 50 KG export worthy Packing

Main Export Market(s)
Western Europe, Africa, Central America, Eastern Europe, Australia, Middle East, South America, Asia, North America

Main Domestic Market
All India

Certifications
ISO 9001:2008, GMP

Eosine Blue About

A number of fluorescent acidic compounds combine together to form Eosin class that helps in binding salts with basic compounds. This may include staining of arginine or lysine protein with the pink or dark red color with the help of bromine and fluorescein. Apart from staining proteins, the mentioned chemical compound may also find usage for staining muscle fibers and collagen to examine them using microscope. The structures that get stained using Eosin are known to be eosinophilic.

Certificate of Analysis :-

Chemical Name : EOSINE BLUE FOR MICROSCOPY
Molecular Formula : C20H6Br2N2Na2O9
M.W. 624
C.I.No. 45400 100 gm
PubChem Substance ID : 329756624
MDL Number : MFCD00005038
H.S. Code : 3204.1200
Dye content(by gravimetry) : min. 85%
Absorption(in water) : max. 517-520 nm
Suitability for microscopy : Passes test
Grade : for microscopy
Shelf Life : 5 Years
Colour : Black Colour powder
Physical State : Solid 20deg C
Un No. NR TARIFF : 3204 90 00
R : 41 S : 22-26 F : 16

Other Applications :-

Used as a background stain by contrasting nuclear stains
For locating the sections of brain
Tissues of liver can be easily stained

High Purity for Reliable Results

Eosine Blue boasts a 99% purity, ensuring consistent staining and reproducibility in laboratory work. Its chemical stability and precise formulation make it an essential reagent for histological, cytological, and molecular biology studies. Supplied in laboratory-grade quality, it meets rigorous analytical reagent standards for reliable academic and research applications.

Versatile Applications in Biological Research

With its principal use in biological staining, Eosine Blue is versatile for a broad range of laboratory protocols, including microscopic analysis of tissues and cells. Its robust staining properties enable clear visualization of cytoplasmic and connective tissue components, making it invaluable for pathologists, cell biologists, and researchers alike.

Safe Handling and Extended Shelf Life

Eosine Blue is not classified as hazardous under normal laboratory conditions, making it user-friendly for daily research activities. It should be stored in a tightly closed container in a cool, dry, and well-ventilated place, away from ignition sources and oxidizers. With a shelf life of 36 months, it remains reliable for long-term use.

FAQ's of Eosine Blue:

Q: How is Eosine Blue used in laboratory staining procedures?

A: Eosine Blue is typically dissolved in water to produce an acid stain solution, which is applied to biological specimens for cytoplasmic and connective tissue staining during microscopy and histological analysis.

Q: What benefits does Eosine Blue provide in biological staining?

A: Eosine Blue delivers vivid, contrasting coloration of cellular and tissue structures, allowing for clear differentiation under the microscope. Its high purity and reliable solubility contribute to reproducible and precise staining results.

Q: When should Eosine Blue be chosen over other laboratory stains?

A: Eosine Blue is ideal for protocols requiring acid staining, particularly for differentiating cytoplasmic elements and connective tissue in histological samples, due to its specific dye properties and compliance with analytical standards.

Q: Where should Eosine Blue be stored for optimal stability?

A: For maximum shelf life and stability, Eosine Blue should be kept in a tightly sealed container, stored in a cool, dry, and well-ventilated area, away from ignition sources and strong oxidizers.

Q: What is the process for preparing Eosine Blue for staining?

A: To prepare Eosine Blue for staining, measure the appropriate amount of powder and dissolve it in distilled water or a suitable solvent, adjusting concentration as per staining protocol before applying to specimens.

Q: Is Eosine Blue hazardous to handle during routine laboratory use?

A: No, Eosine Blue is not classified as hazardous under normal laboratory conditions. Always follow standard laboratory safety guidelines while handling and preparing the stain.

Q: Can Eosine Blue be used for educational as well as research purposes?

A: Yes, Eosine Blue's laboratory grade quality and ease of use make it suitable for both teaching and research environments in universities, colleges, and professional laboratories.

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