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HPLC Buffer Salts

In case of high performance liquid chromatography (HPLC), it is very important to make the selection of the right buffer, as they are used in the mobile phase, and are responsible for holding the buffer constant. HPLC buffer salts are usually made up of a weak acid or weak base, in combination with their with their respective conjugate base/acid in the solution which is usually partially aqueous. The manner in which buffer salts work in the solution is that the aforesaid pair of compounds maintain an equilibrium of sorts, which is why on addition of an acid or a base, a shift in the equilibrium is observed to compensate the change in values, and thus, help in maintenance of a constant pH value.

1) They are commonly used during the analysis of ionizable components in the whole phasing and chromatographic process
2) It is advised that the pH of the buffer should always be at least two (2) values away from the pKa value of the analyte
3) Volatility of the buffer is another is another highly critical parameter which should be duly addressed and modifiers can also be used to keep the pH in check
4) Concentration should ideally be kept at the lowest value at which it can produce viable results as higher concentrations come with a risk of precipitation
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SODIUM DIHYDROGEN ORTHOPHOSPHATE

Price: 938 INR/Gram
  • Appearance:White crystalline powder
  • Application:Used in laboratories and various industrial processes as a buffering agent and reagent
  • Boiling point:Not applicable (decomposes before boiling)
  • Flash Point:Non-flammable
  • Physical State:Solid
  • Purity:99.0%
  • Storage Instructions:Store in tightly closed container, in a cool, dry, well-ventilated area
  • Type:University Lab Chemicals
  • Delivery Time:4-5 Days
  • Supply Ability:50000 Per Week
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SODIUM PHOSPHATE dibasic (dihydrate)

  • Appearance:White crystalline powder
  • Density:1.52 Gram per cubic centimeter(g/cm3)
  • Melting Point:100 C (dehydrates)
  • Molecular Weight:177.99 g/mol
  • Packaging Type:HDPE drum / bags
  • Ph Level:8.9 9.2 (1% solution)
  • Purity(%):99%
  • Usage:Laboratory, industrial, food processing
  • Delivery Time:1 Week
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POTASSIUM HYDROGEN PHTHALATE (potassium biphthalate)

Price: 806 INR/Gram
  • Appearance:WHITE CRYSTALLINE POWDER
  • Density:1.636 Gram per cubic centimeter(g/cm3)
  • Melting Point:240 C
  • Molecular Weight:204.22 g/mol
  • Packaging Type:HDPE Bottle / Polybag
  • Ph Level:~4.00 (0.05 mol/L at 25C)
  • Purity(%):99.5%
  • Usage:Buffer solutions; Calibration of pH meters.
  • Delivery Time:4-5 Days
  • Supply Ability:50000 Per Week
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POTASSIUM PHOSPHATE monobasic (

Price: 1016 INR/Gram
  • Appearance:White Crystalline Powder
  • Application:Laboratory, Industrial, Fertilizers, Food Additives
  • Boiling point:Decomposes before boiling
  • Physical State:Solid
  • Purity:99%
  • Purity(%):99%
  • Storage Instructions:Store in a tightly closed container, in a cool, dry, ventilated area
  • Type:Other, Powder
  • Delivery Time:4-5 Days
  • Supply Ability:50000 Per Week
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SODIUM BICARBONATE (sodium hydrogen carbonate)

Price: 301 INR/Gram

SODIUM HYDROGEN CARBONATE PURIFIED NaHCO3 M.W. 84.01 Minimum assay(by acidimetry) 99.5-101% pH(5% water) 8-8.5 Maximum Limits of Impurities: Chloride(Cl) 0.015% Sulphate(SO4) 0.01% Total nitrogen 0.002% Heavy metals(as Pb) 0.0005% Arsenic(As) 0.0002% Lead(Pb) 0.0005%

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CITRIC ACID (monohydrate) Extra Pure

It is used as a natural preservative and is also used to add an acidic taste to foods & soft drinks. We use optimum grade compounds, sourced from authentic vendors to process this chemical. This chemical also works as an environmentally benign cleaning agent and acts as an antioxidant. We offer our Citric Acid (monohydrate) Extra Pure at pocket friendly prices to clients. It has a tart taste, and is also known as lemon salt or sour salt.

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SODIUM ACETATE (anhydrous)

  • Appearance:White powder or crystals
  • Application:Laboratory reagent, buffer solutions, textile industry, photography, heating pads
  • Boiling point:n/a (decomposes)
  • Physical State:Solid
  • Purity:99%
  • Purity(%):99%
  • Storage Instructions:Store in a tightly closed container, in a cool, dry, well-ventilated place
  • Type:Other, Anhydrous
  • Delivery Time:1 Week
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AMMONIUM PHOSPHATE monobasic AR

It is widely used source of phosphorus and nitrogen. We use finest quality compounds, sourced from trusted vendors to process this chemical. It is used as an ingredient in some fertilizers as a high source of elemental nitrogen. Ammonium Phosphate Monobasic AR is also analytically used as buffer solutions and as flame retardant in thermoplastic compositions. It is employed in the synthesis of the first example of a mixed-valence ammonium iron phosphate. We offer this chemical at affordable prices.

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POTASSIUM PHOSPHATE monobasic

Price: 1016 INR/Gram

It is used as a fertilizer, a food additive and a fungicide. We process this chemical using best quality compounds, sourced from trusted vendors. This chemical is often used as a nutrient source in the greenhouse trade and in hydroponics. For easy transportation, this chemical is packed in moisture proof containers. We offer this Potassium Phosphate Monobasic at cost effective prices to clients. It is used during isolation of protein from bacterial cells and in the preparation of phosphate buffer saline.

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Ammonium Acetate 99%

Price: 510 INR/Gram
  • Application:Laboratory reagent, Analytical chemistry, Pharmaceuticals
  • Boiling point:Decomposes
  • Flash Point:Non Flammable
  • Packaging Type:HDPE Bottles / Drum Pack
  • Physical State:Solid
  • Purity:99%
  • Storage Instructions:Store in a cool, dry place. Keep container tightly closed.
  • Type:University Lab Chemicals
  • Delivery Time:4-5 Days
  • Supply Ability:50000 Per Week
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Ammonium Carbonate

Price: 475 INR/Gram

It is composed of ammonium carbamate and ammonium bicarbonate. We use premium grade compounds, sourced from trusted vendors to process this chemical. It is a leavening agent, used for baking such as cookies and flat biscuits. It is also used in the production of baking soda and baking powder. Packed in moisture proof jars or containers, this chemical is available in different quantity packaging options. We offer Ammonium Carbonate at reasonable prices to clients.

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ALPHA CHEMIKA
GST : 27AAOFA9762F1Z1
ALPHA CHEMIKA
GST : 27AAOFA9762F1Z1
  • Savgan Heights, 102, B Wing, RTO Lane, Andheri (West), Mumbai - 400053, Maharashtra, India
  • Phone : 08045816216
  • Mr Tanmay Mahajan (DIRECTOR)
  • Mobile : 08045816216
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