About Acriflavine for Biochemistry
Acriflavine is a powder which is derived from acridine and appears to be brown or orange in color and works as a topical antiseptic. In biochemistry, it is useful in fluorescent labeling of RNA having high molecular weight. Moreover, this find application for treating fungal infections of fishes that are kept in aquarium. During First World War, it was used to prevent sleeping sickness. Although, the mentioned compound is used for various purposes, however, proper precautions to taken into consideration while using it. If inhaled, Acriflavine may cause irritation to eyes and this dye may stain skin by causing skin irritation.
Certificate of Analysis :-
- Name Of Item : ACRIFLAVINE (for biochemistry)
- Formula : C14H14N3Cl
- M.W. : 259.74
- Cat. No. : AL0104 00500
Technical Specifications :-
| Type Of Test | Standard | Observed |
| | | |
| Description | A red crystalline powder | A red crystalline powder |
| Assay | 98.5-105% w/w | 99.14% |
| Solubility | Complies | Complies |
| Identification tests 1,2,3,4,5 | Complies | Complies |
| Certain other Acridine | | |
| derivatives | Complies | Complies |
| Water | <6.5% w/w | 3.1% w/w |
| Sulphated ash | <1.0% w/w | <1.0% w/w |
Superior Purity for Consistent Laboratory ResultsAcriflavine stands out with its high assay value of over 99%, minimal impurities (<0.5%), and consistent crystalline red appearance. Its reliable purity level (99.14%) ensures precision in biochemical research, staining, and antimicrobial experiments, critical for reproducible laboratory outcomes.
Versatility in Research and ApplicationThis compound acts as a potent dye, antimicrobial, and biochemical reagent, making it ideal for staining cell structures, antimicrobial testing, and various research protocols. Its broad range of laboratory uses caters to both academic and industrial research needs, delivered via customized packaging for convenience.
Safe Handling and Optimal StorageAcriflavine should be handled with care as it is designated an irritant. Laboratory personnel must use appropriate PPE and store the substance in a cool, dry, dark place. The sealed packaging preserves its integrity for up to 36 months, ensuring stability as approved by quality standards.
FAQ's of Acriflavine for Biochemistry:
Q: How should Acriflavine be handled safely in the laboratory?
A: Acriflavine is classified as an irritant; always use personal protective equipment such as gloves, lab coats, and safety goggles. Avoid inhalation or direct skin contact. Work in a well-ventilated environment and handle with caution, following hazardous material guidelines.
Q: What is the recommended process for dissolving Acriflavine?
A: Acriflavine is soluble in both water and ethanol. For laboratory use, add the desired amount to the solvent and stir until fully dissolved. Prepare solutions fresh when possible and protect from light throughout the process to maintain stability.
Q: Where should Acriflavine be stored for optimal stability?
A: Store the product in a tightly sealed bottle or vial in a cool, dry location away from direct light, as the compound is light sensitive. Under these conditions, Acriflavine remains stable and effective for up to 36 months.
Q: What is the primary usage of Acriflavine in biochemical research?
A: Acriflavine is mainly used as a staining agent and antimicrobial compound in biochemical laboratories. It helps visualize cellular structures and test antimicrobial activity in microbiological experiments.
Q: When is Acriflavine most beneficial in laboratory applications?
A: Acriflavine is especially advantageous when high contrast staining or reliable antimicrobial effects are required in research. Its high purity and consistent crystalline form ensure reproducibility in sensitive experiments.
Q: How does the molecular composition of Acriflavine contribute to its function?
A: With a molecular formula of C14H14ClN3HCl and a molecular weight of 370.4 g/mol, Acriflavine's structure supports its effective binding as a dye and its antimicrobial properties, making it valuable for both staining and biological testing protocols.