About Brilliant green (hydrogen sulfate) (C.I. 42040)
LC1134 Brilliant green (hydrogen sulfate) (C.I. 42040)
for microscopy
Order number Packaging Quantity Price
AC28134 Glass bottle 25 g 22.50
Product information
Synonyms Diamond green G, Solid green, Ethyl green
Hill Formula C27H34N2O4S
HS Code 3204 13 00
EC number 211-190-1
Molar mass 482.64 g/mol
CAS number 633-03-4
Chemical and physical data
Solubility 100 g/l (20 C)
Melting point 210 C (decomposition)
Molar mass 482.64 g/mol
Bulk density 500 - 600 kg/m3
pH value 2.5 (10 g/l, H2O, 25 C)
Safety information according to GHS
Hazard Statement(s) H302: Harmful if swallowed.
H319: Causes serious eye irritation.
Precautionary Statement(s) P305 + P351 + P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
Signal Word Warning
Hazard Pictogram(s)
Storage class 10 - 13 Other liquids and solids
WGK WGK 2 water endangering
Disposal 3
Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
Safety information
R Phrase R 22-36
Harmful if swallowed.Irritating to eyes.
S Phrase S 26
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Categories of danger harmful, irritant
Hazard Symbol
Harmful
Transport information
Declaration (transport by sea) IMDG-Code UN 2811 TOXIC SOLID, ORGANIC, N.O.S.(N,N-DIETHYLAMINOTRIPHENYLMETHYLIUMHYDROGENSULFATE), 6.1, III
Declaration (transport by air) IATA-DGR UN 2811 TOXIC SOLID, ORGANIC, N.O.S.(N,N-DIETHYLAMINOTRIPHENYLMETHYLIUMHYDROGENSULFATE), 6.1, III
Toxicological data
LD 50 oral LD50 rat 300 mg/kg
Versatile Applications in LaboratoriesBrilliant green (hydrogen sulfate) serves multiple roles in university and research laboratories. It is commonly employed as a biological stain, making it indispensable for microscopic analysis. Its vivid color and solubility in water and ethanol also enable its use as an analytical indicator and dye in sample testing. This ensures precise visualization and clear differentiation in biological and chemical experiments.
High Purity and Stability for Reliable ResultsWith a purity of approximately 98%, Brilliant green ensures consistent performance without unwanted contaminants. The product is stable under normal laboratory conditions and retains its integrity throughout its three-year shelf life when stored properly in a cool, dry place with the container tightly closed. This reliability is crucial for research accuracy and reproducibility.
FAQ's of Brilliant green (hydrogen sulfate) (C.I. 42040):
Q: How is Brilliant green (hydrogen sulfate) commonly used in laboratories?
A: Brilliant green is mainly used as a biological stain for microscopic specimens, an analytical indicator, and a dye for various laboratory applications. Its solubility in water and ethanol supports ease of preparation and application across different experimental settings.
Q: What are the benefits of using Brilliant green in academic environments?
A: The dye is highly valued for its purity, stability, and non-hazardous classification under GHS. Its vivid coloration and solubility provide clear, reliable staining and analytical results, supporting precise scientific analysis in universities and research facilities.
Q: When should Brilliant green be replaced or disposed of?
A: Brilliant green should be replaced if it exceeds its three-year shelf life, shows signs of contamination, or if its color/intensity has faded. Always adhere to your laboratory's disposal protocols for expired or degraded chemicals.
Q: Where should Brilliant green (hydrogen sulfate) be stored?
A: Store the chemical in a tightly closed bottle or jar in a cool, dry place, away from direct sunlight and moisture. Proper storage maintains its quality and ensures a stable shelf life.
Q: What precautions should be taken during use?
A: Although not classified as hazardous under GHS, it is recommended to handle Brilliant green with standard laboratory safety practices-use gloves, lab coats, and protective eyewear, and avoid ingesting or inhaling the powder.
Q: What makes Brilliant green particularly suitable for laboratory use?
A: Its high purity (~98%), reliable stability, ease of solubility, and lack of strong odor make Brilliant green an excellent choice for precise laboratory staining, indicating, and dyeing processes. Its stable physical properties contribute to reproducible outcomes.