About Eosine Blue
A number of fluorescent acidic compounds combine together to form Eosin class that helps in binding salts with basic compounds. This may include staining of arginine or lysine protein with the pink or dark red color with the help of bromine and fluorescein. Apart from staining proteins, the mentioned chemical compound may also find usage for staining muscle fibers and collagen to examine them using microscope. The structures that get stained using Eosin are known to be eosinophilic.
Certificate of Analysis :-
- Chemical Name : EOSINE BLUE FOR MICROSCOPY
- Molecular Formula : C20H6Br2N2Na2O9
- M.W. 624
- C.I.No. 45400 100 gm
- PubChem Substance ID : 329756624
- MDL Number : MFCD00005038
- H.S. Code : 3204.1200
- Dye content(by gravimetry) : min. 85%
- Absorption(in water) : max. 517-520 nm
- Suitability for microscopy : Passes test
- Grade : for microscopy
- Shelf Life : 5 Years
- Colour : Black Colour powder
- Physical State : Solid 20deg C
- Un No. NR TARIFF : 3204 90 00
- R : 41 S : 22-26 F : 16
Other Applications :-
- Used as a background stain by contrasting nuclear stains
- For locating the sections of brain
- Tissues of liver can be easily stained
High Purity for Reliable ResultsEosine Blue boasts a 99% purity, ensuring consistent staining and reproducibility in laboratory work. Its chemical stability and precise formulation make it an essential reagent for histological, cytological, and molecular biology studies. Supplied in laboratory-grade quality, it meets rigorous analytical reagent standards for reliable academic and research applications.
Versatile Applications in Biological ResearchWith its principal use in biological staining, Eosine Blue is versatile for a broad range of laboratory protocols, including microscopic analysis of tissues and cells. Its robust staining properties enable clear visualization of cytoplasmic and connective tissue components, making it invaluable for pathologists, cell biologists, and researchers alike.
Safe Handling and Extended Shelf LifeEosine Blue is not classified as hazardous under normal laboratory conditions, making it user-friendly for daily research activities. It should be stored in a tightly closed container in a cool, dry, and well-ventilated place, away from ignition sources and oxidizers. With a shelf life of 36 months, it remains reliable for long-term use.
FAQ's of Eosine Blue:
Q: How is Eosine Blue used in laboratory staining procedures?
A: Eosine Blue is typically dissolved in water to produce an acid stain solution, which is applied to biological specimens for cytoplasmic and connective tissue staining during microscopy and histological analysis.
Q: What benefits does Eosine Blue provide in biological staining?
A: Eosine Blue delivers vivid, contrasting coloration of cellular and tissue structures, allowing for clear differentiation under the microscope. Its high purity and reliable solubility contribute to reproducible and precise staining results.
Q: When should Eosine Blue be chosen over other laboratory stains?
A: Eosine Blue is ideal for protocols requiring acid staining, particularly for differentiating cytoplasmic elements and connective tissue in histological samples, due to its specific dye properties and compliance with analytical standards.
Q: Where should Eosine Blue be stored for optimal stability?
A: For maximum shelf life and stability, Eosine Blue should be kept in a tightly sealed container, stored in a cool, dry, and well-ventilated area, away from ignition sources and strong oxidizers.
Q: What is the process for preparing Eosine Blue for staining?
A: To prepare Eosine Blue for staining, measure the appropriate amount of powder and dissolve it in distilled water or a suitable solvent, adjusting concentration as per staining protocol before applying to specimens.
Q: Is Eosine Blue hazardous to handle during routine laboratory use?
A: No, Eosine Blue is not classified as hazardous under normal laboratory conditions. Always follow standard laboratory safety guidelines while handling and preparing the stain.
Q: Can Eosine Blue be used for educational as well as research purposes?
A: Yes, Eosine Blue's laboratory grade quality and ease of use make it suitable for both teaching and research environments in universities, colleges, and professional laboratories.