About EVAN S BLUE (for microscopy)
EVANS BLUE FOR MICROSCOPY
C34H24N6Na4O14S4 M.W. 960.82 C.I. 23860
Assay min. 90%
Absorption maxima 606-611
Absorptivity 930-1040
(A 1%/cm Absop max. ; 0.0006%; water)
Loss on drying(110oC) max 7.0%
Suitability for microscopy passes test
For estimation of blood volume
CAS No. 314-13-6 Un No. NR TARIFF : 3204 90 00
Advanced Staining Performance for Research LabsEvan's Blue offers robust staining capabilities crucial for detailed microscopy and histological analysis. Its high dye content ensures vibrant, consistent results, making it indispensable for academic research, biological studies, and laboratory protocols. The dye's pronounced absorption at 610 nm and 630 nm provides vivid contrast, enhancing cellular and tissue visualization under the microscope.
Key Storage and Safety ConsiderationsProper storage is essential for maintaining Evan's Blue's quality. The dye is sensitive to light and should be stored in tightly sealed containers, away from direct sunlight, in a cool and dry environment. While non-flammable, it should be handled using appropriate personal protective equipment (PPE) to avoid contact. Following recommended storage greatly extends shelf life, which can reach up to five years under ideal conditions.
FAQ's of EVAN S BLUE (for microscopy):
Q: How should Evan's Blue be prepared for use in microscopy applications?
A: To prepare Evan's Blue for microscopy, dissolve the powder in ethanol for optimal solubility, or in water if needed, to create the required staining solution. Ensure solutions are freshly prepared and handle under subdued lighting to minimize degradation due to light sensitivity.
Q: What benefits does Evan's Blue provide in histology and biological staining?
A: Evan's Blue offers high dye content and uniform composition, resulting in dependable, vivid staining for microscopic and histological examination. Its specific absorption maxima confer strong visualization, enhancing the contrast of biological samples.
Q: When and how should Evan's Blue be stored to maintain its quality?
A: Store Evan's Blue in a tightly closed container, in a cool, dry area shielded from light exposure. Proper storage preserves dye potency and guarantees a shelf life of up to five years, ensuring reproducibility in research and diagnostics.
Q: Where is Evan's Blue suitable for use, and who supplies it?
A: Evan's Blue is ideal for use in university laboratories, research centers, and biological labs throughout India. It is available from recognized dealers, exporters, manufacturers, and laboratory suppliers nationwide.
Q: What precautions should be taken when handling Evan's Blue?
A: Although non-flammable, Evan's Blue requires handling with appropriate PPE, such as gloves, goggles, and lab coats, to prevent skin or eye contact. Always work in a well-ventilated area and avoid exposure to light during handling and storage.
Q: Can Evan's Blue be used in solutions of varying pH?
A: Evan's Blue exhibits a neutral pH (6.0-7.0) in a 1% solution, making it compatible with most staining protocols that require a near-neutral environment. This helps maintain sample integrity during microscopic and histological analysis.