About ORCEIN (for microscopy)
CERTIFICATE OF ANALYSIS
| Name Of Item : ORCEIN (for microscopy) | | Formula : --- |
| M.W. : --- | | Batch No. : O0062 |
| CAS NO. : 1400-62-0 | | | Cat. No. : AL3355 00010 |
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| Type Of Test | Standard | | Observed |
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| Description | Black coloured powder | | Black coloured powder |
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| Absorption maxima (in 0.01N | | |
| NaOH) | 575 582 nm | | 579 nm |
| Spec. Absorptivity A1%/1cm ( max, 0.001%, | | |
| 0.01N NaOH) | Min. 270 | | 287 |
| Loss on drying | <7.0% | | 4.9% |
| TLC Test | Passes test | | Passes test |
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| Results : The above product complies with LR grade | | |
Versatile Microscopy StainORCEIN is a trusted choice for both routine and specialized microscopic staining applications, including histology and cytology. Its high quality and laboratory-grade purity ensure reproducible results across a range of specimen types. The partially soluble nature facilitates controlled staining, making it suitable for academic, clinical, or research laboratories.
Safe and Reliable Laboratory UseWith no hazardous classification under normal laboratory operations, ORCEIN can be used confidently by laboratory personnel. Proper storage-keeping the bottle or jar tightly closed in a cool, dry location-helps maintain the powder's efficacy over its minimum 24-month shelf life. Wide availability in multiple pack sizes adds to its adaptability.
FAQ's of ORCEIN (for microscopy):
Q: How should ORCEIN be prepared and used for microscopy staining?
A: To prepare an ORCEIN staining solution, dissolve the powder in water or ethanol to achieve a concentration between 0.1% and 1% (w/v) as recommended. Stir the solution thoroughly to ensure as much dissolution as possible, although complete solubility may not be achieved. Apply the solution to biological specimens as per your staining protocol, and proceed with microscopic observation.
Q: What types of specimens or applications is ORCEIN suitable for?
A: ORCEIN is suitable for staining a variety of biological specimens in microscopy and histology. It is commonly used to highlight chromosomes, elastic fibers, and other cellular structures, making it valuable in educational, research, and clinical settings.
Q: When should I replace my ORCEIN stock solution or powder?
A: ORCEIN powder maintains its performance for at least 24 months if stored per guidelines. Replace the powder if you notice significant color changes, clumping, or if the solution's staining efficiency decreases. Prepared solutions should be used within a reasonable time frame and discarded if contamination is suspected.
Q: Where is the best place to store ORCEIN powder?
A: Store ORCEIN powder in a tightly closed container, kept in a cool, dry place away from direct sunlight. Proper storage conditions help preserve the dye's quality and extend its usable shelf life.
Q: What are the benefits of using ORCEIN for laboratory staining?
A: ORCEIN provides clear and distinct staining of target structures, with ease of preparation and application. Its moderate pH range and non-hazardous nature make it user-friendly and safe for routine laboratory protocols.
Q: Is ORCEIN hazardous to handle in standard laboratory environments?
A: ORCEIN is not classified as hazardous for standard laboratory use. Normal safety precautions-such as wearing gloves and using personal protective equipment-are recommended, but special hazard controls are generally unnecessary.
Q: What packaging sizes are available for ORCEIN, and how can I select the right one?
A: ORCEIN is available in various pack sizes, including 10g, 25g, and 100g bottles or jars. Select a packaging size based on your expected frequency of use and storage capacity, ensuring you use the powder within its shelf life for optimal results.