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Crystal Violet

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MOQ : 25GM Kilograms

Crystal Violet Specification

Molecular Formula
C25H30N3Cl

Physical State
Solid powder

Purity
> 99%

Melting Point
205-207C

Size
25g / 100g / 500g

Ph Level
~3.0 (1% aqueous solution)

Usage
Used as a biological stain and pH indicator

Molecular Weight
407.98 g/mol

Density
1.0 Gram per cubic centimeter(g/cm3)

Shelf Life
5 years (if unopened and properly stored)

Packaging Type
HDPE bottle / Glass bottle

Boiling point
Decomposes before boiling

Storage Instructions
Store in a cool, dry place away from light

CAS No
548-62-9

Grade
Laboratory Grade

Type
Powder Dye

Application
Biological staining, microbiology, laboratory reagents

Appearance
Dark purple crystalline powder

Purity(%)
> 99%

UN Number
3077

Refractive Index
1.686

HS Code
32041399

pKa value
1.85

Solubility
Freely soluble in water and ethanol

Stability
Stable under recommended storage conditions

Odor
Odorless

Toxicity
Harmful if swallowed or inhaled, suspected carcinogen

EC Number
208-953-6

Hazard Class
Class 9 (Miscellaneous Dangerous Substances)

Color Index
42555

Moisture Content
<0.5%

Crystal Violet Trade Information

Minimum Order Quantity
25GM Kilograms

Delivery Time
1 Week

Sample Available
Yes

Sample Policy
Sample costs shipping and taxes has to be paid by the buyer

Packaging Details
g

Main Export Market(s)
North America, Eastern Europe, Middle East, Western Europe, Africa, Central America, Australia, South America, Asia

Main Domestic Market
All India

Certifications
1 Packet contains 100 strip

About Crystal Violet

Crystal Violet is also called as gentian violet and methyl violet 10B, which is known tobe a historical stain for classifying bacteria. The chemical formula, molecular weight and melting point of the mentioned organic compound is C25N3H30Cl, 407.979 g/mol and 205 degree Celsius, respectively. Offered compound appears to be blue-violet in color, when it is dissolved in water. It is highly demanded in the chemical industry and pharmaceuticals because of its anti-bacterial, anthelmintic and anti-fungal properties.

Certificate of Analysis :-

Name : CRYSTAL VIOLET FOR MICROSCOPY CRYSTAL
Methyl Violet : 1OB C I Basic Violet 3
Synonym : Basic Violet 3, Methyl Violet 10B, Gentian Violet, Hexamathylpararosaniline
IUPAC Name : Tris(4-(dimethylamino)phenyl)methylium
Molar Mass : 407.979 g/mol
Formula : C25N3H30Cl
Density : 1.19 g/cm cube
EC Number : 208-953-6
MDL Numbar : MFCD00011750
PubChem Substance ID : 57654029
C.I. No. : 42555
MP : 401 Fdeg
Product Description : Cation based violet dye
Assay : 96.0 100.5%
Identification test To pass A, B & C tests
Water NMT : 7.5%
Residue on ignition NMT : 1.5%
Alcohol-insoluble substances NMT : 1.0%
Arsenic NMT : 0.001%
Lead NMT : 0.003%
Zinc Passes test
Chromatographic purity : Passes test
Un No : 9/3077
TARIFF : 3204 13 49

Applications :-

Its components are used for inking ball point pens, ink jet printers, etc.
Fertilizers, detergents, leathers, anti-freezers can be easily colored
Marking is made on skin during the preparation of surgery
In paper and print industry, it helps in dying paper

Versatile Laboratory Dye

Crystal Violet stands as a preferred choice for microbiologists and lab technicians due to its high purity and effectiveness in biological staining and pH indication. Its unique coloration and reliable results make it essential for diagnostic laboratories, educational purposes, and research centers.

Safety and Storage Guidelines

As a Class 9 hazardous substance, Crystal Violet should be handled with caution. Store the powder in a cool, dry area away from direct sunlight, using HDPE or glass bottles to maintain its stability and shelf life. Proper storage ensures product integrity and safety in the laboratory environment.

Applications and Usage

Crystal Violet is widely used in Gram staining for microbiology, histology, and as a chemical indicator in laboratories. Its reliable staining properties enhance cell visualization and support accurate laboratory results, benefiting both research and diagnostic applications.

FAQ's of Crystal Violet:

Q: How should Crystal Violet be stored to maintain its stability and effectiveness?

A: Crystal Violet should be kept in a cool, dry place away from light, preferably in HDPE or glass bottles. This ensures stability and preserves its 5-year shelf life when unopened and stored under proper conditions.

Q: What are the common applications of Crystal Violet in laboratories?

A: Crystal Violet is primarily used as a biological stain in microbiology and histology, and also serves as a pH indicator. It facilitates cell visualization, microbial identification, and assists in various research protocols.

Q: When is it appropriate to use Crystal Violet as a pH indicator?

A: Crystal Violet is suitable as a pH indicator in solutions with a pH near 3.0, where it displays reliable color changes and sensitivity that assist with accurate pH measurements in experimental and analytical procedures.

Q: Where can Crystal Violet be used in the scientific process?

A: Crystal Violet is used in staining bacterial cells during the Gram staining process, and in histological studies for visualizing cellular components. It can also be applied in research and educational labs across India.

Q: What safety precautions should be observed when handling Crystal Violet?

A: Due to its toxic and possibly carcinogenic nature, users should avoid inhalation, ingestion, and direct skin contact. Personal protective equipment like gloves, masks, and eye protection are recommended during handling.

Q: How is Crystal Violet beneficial for scientific analysis?

A: Crystal Violet offers precise and vibrant staining of biological samples, improving visibility under a microscope. Its reliable performance supports high-quality, reproducible results in clinical, educational, and research settings.

Q: What is the process of dissolving Crystal Violet for laboratory use?

A: Crystal Violet is freely soluble in water and ethanol. Simply add the required amount of powder to the solvent and mix until fully dissolved, creating a ready-to-use staining or indicator solution for laboratory tests.

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