About Fluorescein Sodium
Technical Specifications :-
- Product Name : Fluorescein Sodium
- Synonym : Acid yelow 73, NsFluo, Uranine, Sodium Fluorescein
- CAS No. 518-47-8
- C.I. No. : 45350 100 gm
- EC NO. : 208-253-0
- MDL NO. : MFCD00167039
- PubChem Substance ID : 24894907
- Un No. NR TARIFF : 3204 90 00
- Empirical Formula : C20H10Na2O5
- M.W. : 376.28 g/mol
- Color : Orange red to dark red
- Odor : Odorless
- pH (5% solution) : 7.8
- Vapor Pressure : Negligible
- Minimum Assay : 1 kg (HClO4 titration)
Maximum Limits of Impurities :-
- Lead(Pb) 0.002%
- Copper(Cu) 0.01%
- Cadmium(Cd) 0.001%
- Zinc(Zn) 0.01%
- Arsenic(As) 0.0002%
- Water(K. Fischer) 7.0%
- Acriflavine passes test
Exceptional Laboratory VersatilityDue to its strong fluorescence profile and chemical stability, Fluorescein Sodium is widely used in research, clinical diagnostics, and industrial processes. Its versatility as a biological stain and tracer provides precise detection, making it indispensable for a range of scientific and medical analyses.
Reliable and Stable PerformanceWith a remarkable shelf life of up to 5 years under recommended storage conditions, Fluorescein Sodium ensures consistent results. Its resistance to light-induced degradation, coupled with non-flammable properties, guarantees both safety and integrity during storage and application.
Optimal Application and HandlingFluorescein Sodium is conveniently offered in both glass and plastic bottles, with custom packaging available to suit various user demands. Its ease of solubility and pH range (5.0-7.5 in a 1% solution) makes it easy to prepare and use across diverse protocols, further simplifying laboratory workflows.
FAQ's of Fluorescein Sodium:
Q: How should Fluorescein Sodium be stored to maintain its stability?
A: Fluorescein Sodium should be kept in a tightly closed container at room temperature, protected from direct light, to ensure long-term stability and effectiveness throughout its shelf life of five years.
Q: What are the main applications of Fluorescein Sodium?
A: This compound is primarily used as a biological stain in microscopy, a diagnostic agent in medical testing (such as eye exams), and as a fluorescent tracer in laboratory research due to its strong fluorescence properties.
Q: When is Fluorescein Sodium most effectively used in laboratory procedures?
A: Fluorescein Sodium offers optimal performance when dissolved in water, taking advantage of its strong absorption (494 nm) and emission (521 nm) characteristics. It is commonly used in protocols requiring fluorescent tracking or visualization.
Q: Where is Fluorescein Sodium typically utilized?
A: Fluorescein Sodium is widely employed in hospitals, diagnostic laboratories, research institutions, and scientific facilities throughout India and globally, wherever precise fluorescent detection is needed.
Q: What process is recommended for preparing Fluorescein Sodium solutions?
A: To prepare a working solution, dissolve the powdered dye in distilled water until fully dissolved. The typical pH range for a 1% solution is between 5.0 and 7.5, suitable for most biological and analytical applications.
Q: How is Fluorescein Sodium beneficial in diagnostic procedures?
A: Its intense fluorescence under specific lighting allows for high-contrast visualization, particularly useful in medical imaging, detecting leaks, or highlighting biological specimens, improving analytical accuracy.
Q: What makes Fluorescein Sodium a preferred choice for laboratory-grade experiments?
A: The combination of high purity (98%), robust stability, strong fluorescence, ease of handling, and customizable packaging ensures reliable performance across a broad spectrum of scientific uses.