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Auramine for Microscopy

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MOQ : 25GM Kilograms

Auramine for Microscopy Specification

Melting Point
120C

Ph Level
Neutral

Packaging Type
Bottle

Purity
>95%

Size
25 g, 100 g, 500 g

Shelf Life
36 months

Molecular Weight
303.83 g/mol

Storage Instructions
Store in a cool, dry place, protected from light

Molecular Formula
C17H22ClN3

Usage
Microscopic examination, acid-fast staining

Physical State
Powder

CAS No
2465-27-2

Grade
Laboratory Grade

Type
Staining reagent

Application
Microscopy, Fluorescent staining

Appearance
Yellow colored powder

Purity(%)
>95%

Solubility
Soluble in water and ethanol

Hazard Statements
Irritant

Odor
Odorless

Recommended Concentration
0.11% (w/v) for staining

HS Code
32041200

Lead Time
Ready stock

pH of Working Solution
7.0

Color Index Number
CI 41000

Chemical Name
Auramine O

Auramine for Microscopy Trade Information

Minimum Order Quantity
25GM Kilograms

Delivery Time
1 Week

Sample Available
Yes

Sample Policy
Sample costs shipping and taxes has to be paid by the buyer

Packaging Details
500GM, 1KG, 2.5KG, 5KG, 25KG, 50 KG export worthy Packing

Main Export Market(s)
Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa, Asia

Main Domestic Market
All India

Certifications
ISO 9001:2008, GMP

About Auramine for Microscopy

Auramine is known to be diarylmethane dye which acts as a version of Schiff reagent and its crystals appear to be yellow in color in their pure form. It is soluble in water and ethanol. Being a fluorescent stain in nature, our offered Auramine plays a crucial role in the process of microscopy with its staining capabilities of detecting acid-fast bacteria called Mycobaterium. During microscopy, possibility of artifacts can be minimized by the counter stain potassium permanganate. Thus, the cells viewed appear to be reddish orange or bright yellow in color under ultraviolet light. When it combines with Rhodamine, mycolic acids present in cell wall of various organisms helps in providing resistance against de-colorization with the help of acid-alcohol.

Certificate of Analysis :-

Name Of Item : AURAMINE (M.S.) (C.I. No. 41000)
Formula : C17H22N3Cl
M.W. : 303.84
Batch No. : A0580
Cat. No. : AL0511 00100

Technical Specifications :-

Type Of Test	Standard	Observed
Description	Yellow colored powder	Yellow colored powder
Absorption	Max. 431-433nm	432 nm
Absorptivity ( A1%;
1cm=432 nm , pH 6.0 on
dried substance)	880 - 940	931.6
Loss on drying ( 105C )	< 2%	1.2%
Suitability for microscopy	Passes test	Passes test

Results : The above product complies with LR grade

Versatile Staining Reagent for Reliable Microscopy

Auramine O delivers high-contrast fluorescent staining for precise microscopic examinations, especially acid-fast bacteria detection. Its excellent solubility in water and ethanol ensures ease of preparation, while its neutral pH maintains sample integrity. Suited for use by research laboratories and exporters, its stability and purity make it a valuable tool for advanced and routine laboratory work.

Simple Storage and Safe Handling

This reagent is supplied as a stable yellow powder in sealed bottles of various sizes. For best results, store Auramine O in a cool, dry environment and protect it from light. Although it is classified as an irritant, safe handling with appropriate protective gear ensures straightforward usage. Clearly-labeled packaging assists in proper identification and storage.

FAQ's of Auramine for Microscopy:

Q: How should Auramine O be stored for optimal shelf life?

A: Store Auramine O in a cool, dry place away from direct light. This ensures it remains stable and maintains its effectiveness throughout its 36-month shelf life.

Q: What is the recommended concentration of Auramine O for staining?

A: For microscopy and fluorescent staining, dilute Auramine O to a working solution in the range of 0.1-1% (w/v). Prepare fresh solutions as needed to guarantee optimal staining results.

Q: When is Auramine O used in laboratory microscopy?

A: Auramine O is typically used during microscopic examination and acid-fast staining procedures, particularly for detecting mycobacteria and certain fungi in clinical and research laboratories.

Q: Where can Auramine O be sourced and in what packaging sizes?

A: Auramine O is available from dealers, exporters, manufacturers, retailers, suppliers, and traders across India. It is supplied in 25 g, 100 g, and 500 g bottles for laboratory use.

Q: What precautions should be taken while using Auramine O?

A: As Auramine O is classified as an irritant, you should handle it with gloves, protective clothing, and in a well-ventilated area. Avoid inhalation and skin contact to maintain safety during use.

Q: How is Auramine O prepared for microscopic staining?

A: To prepare a staining solution, dissolve the powder in distilled water or ethanol until the desired concentration (0.1-1% w/v) is achieved. Make sure the working solution is neutral (pH 7.0) for optimal staining performance.

Q: What are the main benefits of using Auramine O for microscopy?

A: Auramine O offers high-purity, effective fluorescent staining and excellent solubility, ensuring vibrant visualization under the microscope. Its stability and compatibility with standard laboratory protocols make it a reliable choice for detailed microscopic analysis.

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