About Aniline Blue (Spirit Soluble) for Microscopy
Aniline Blue which is soluble in spirit is also known by the name Soluble Blue or China Blue. It is prepared by combining water blue and methyl blue in an appropriate proportions. This can also be offered in its concentrated form without blending any chemicals. The chemicals involved are diphenyl rosaniline, triphenyl rosaniline and trisulfonates. This is works as a dye which is highly demanded as a biological dye in various industrial sectors such as research development, laboratories, etc. During Fluorescence Microscopy, under violet light, it appears to be yellow-green in color. Our offered Aniline Blue helps in staining collagen in Masson's trichome and plant tissues.
Certificate of Analysis :-
- Name Of Item : METHYL BLUE (C.I. No. 42780)
- Formula : C37H27N3Na2O9S3
- M.W. : 799.81
- Batch No. : M0297
- Cat. No. : AL2539
- Article No : 01381
- H.S. Code : 3204.1251
- Absorption maximum in methanol : 591 - 595nm
- Grade : for Microscopy
- Shelf Life : 5 years
- Appearance : Reddish purple to black powder
- Odour : Odorless
Technical Specifications :-
| Type Of Test | Standard | Observed |
| Description | Bluish black powder | Bluish black powder |
| Absorption Maxima (in | | |
| 0.005M, HCl) | 595 "605 nm | 603 nm |
| Specific absorptivity (in | | |
| 0.005M, HCl) | 675 - 1125 | 680 |
| Loss on drying at 110 C | <7.0% | 5.5% |
| Suitability for microscopy | Passes test | Passes test |
Results : The above product complies with LR grade
Highly Specific Staining PropertiesAniline Blue (Spirit Soluble) is renowned for its selective staining ability, highlighting cellulose in plant cell walls and connective tissue fibers. Its high level of purity ensures consistent and accurate staining results, making it a preferred choice for researchers and laboratory technicians in both biochemistry and molecular biology applications. This dye improves visualization of microscopic structures, aiding in more precise diagnostics and analyses.
Safe and Simple to UseWith minimal toxicity and an odourless nature, Aniline Blue is user-friendly for laboratory settings. The powder can be swiftly dissolved in either water or alcohol, making solution preparation straightforward. As standard laboratory precautions suffice for safe handling, this reduces the risks associated with exposure. Recharge the dye's effectiveness by keeping it in a tightly closed container, away from light and moisture.
Versatile Packaging and Long Shelf LifeAvailable in convenient 10g, 25g, 50g, and 100g packs, Aniline Blue is suited for both small-scale and large laboratory needs. Its robust packaging preserves dye integrity, and unopened containers provide a shelf life of up to three years. This ensures you always have a reliable supply for frequent or occasional microscopy and staining tasks.
FAQ's of Aniline Blue (Spirit Soluble) for Microscopy:
Q: How is Aniline Blue (Spirit Soluble) typically used in microscopy?
A: Aniline Blue is primarily utilized as a special stain for highlighting plant cell walls, cellulose, and connective tissue fibers in biological samples. Its high solubility in spirit and water ensures quick preparation and effective staining for microscopic observations.
Q: What benefits does this dye offer for laboratory and diagnostic applications?
A: Because of its high purity (approx. 99%) and staining specificity, Aniline Blue delivers clear and consistent staining results, making it ideal for analytical, diagnostic, and research tasks where highlighting cellular structures is crucial.
Q: When should I use Aniline Blue (Spirit Soluble) over other dyes?
A: You should use Aniline Blue when you require detailed visualization of cellulose, plant cell walls, or connective tissue fibers, particularly in botanical and histological studies. Its specific affinity for these components makes it preferable to general-purpose dyes in these instances.
Q: Where should Aniline Blue be stored for optimal stability?
A: For best results, Aniline Blue should be stored in a tightly sealed container, protected from light, in a cool, dry place. Proper storage maintains dye integrity and extends its shelf life to up to three years in unopened packaging.
Q: What is the process for preparing Aniline Blue staining solutions?
A: Simply dissolve the required amount of powder in water or alcohol, depending on your staining protocol. The dye's high solubility facilitates rapid preparation of staining solutions for immediate use in microscopy.
Q: Are there any safety precautions required when handling this product?
A: Although the toxicity is low, Aniline Blue can irritate eyes, skin, or the respiratory system. Standard laboratory safety precautions-such as wearing gloves, goggles, and working in a well-ventilated area-are recommended during use.
Q: What packaging sizes are available for purchase?
A: Aniline Blue is available in 10g, 25g, 50g, and 100g pack sizes, catering to diverse research and diagnostic requirements from small-scale experiments to larger batch staining tasks.